Journal: eFood

Article Title: Docosahexaenoic Acid and Nervonic Acid Synergically Enhance Cognitive Memory in Normal Mice via Brain Fatty Acids Remodeling

doi: 10.1002/efd2.70059

Figure Lengend Snippet: FIGURE 3 | DHA and NA enhanced c‐Fos expression in the hippocampus of normal mice. (A) Representative c‐Fos immunopanned photos in the hippocampus. (B) The number of c‐Fos+ neurons in the hippocampal DG area. (C) The expression of PSD95 in the hippocampus. (D) The expression of synaptophysin (SYP) in the hippocampus. *p < 0.05, **p < 0.01 vs control group. One‐way ANOVA post‐hoc Dunnett's test (mean ± SEM, n = 4).

Article Snippet: Immunostaining was performed using monoclonal antibodies against c‐Fos (1:1000; Cell Signaling Technology, cat#2250S) and synaptophysin (SYP, 1:400; Santa Cruz, sc‐17750), polyclonal antibody against PSD95 (1:200; Invitrogen, cat#51‐6900), followed by incubation at 4°C for 24 h. Goat anti‐ mouse (Alexa Fluor 594, 1:500, ab150116) and goat anti‐rabbit (Alexa Fluor 488, 1:500, ab150077) IgG were used as secondary antibodies and incubated in the dark at room temperature for 2 h. Fluorescent images were captured using an ECHO Revlove fluorescence microscope (ECHO, San Diego, California, USA) and quantitatively analyzed using ImageJ software (NIH, Bethesda, Maryland, USA).

Techniques: Expressing, Control

Journal: Brain, behavior, and immunity

Article Title: Rats bred for low intrinsic aerobic exercise capacity link obesity with brain inflammation and reduced structural plasticity of the hippocampus.

doi: 10.1016/j.bbi.2021.06.017

Figure Lengend Snippet: Fig. 4. Older rats expressed less synaptic proteins (arbitrary units) in the hippocampus compared to young rats and the difference is more drastic in low-capacity Runner (LCR) rats than in high- capacity runner (HCR) rats. Western blot analyses of the expression of (A) synapsin (SYN-1) and (B) synaptophysin (SYP) in hippocampal homogenates of HCR and LCR rats at the age of 8 weeks (young) and 10 months (old). The membrane was cut horizontally to separate SYN-1 and SYP according to their molec ular weights, which are shown with the representative blot image. The graphs show the amount of the pro teins normalized to the total protein as analyzed from the stain-free blot. From the stain-free blot only the band of approximately 50 kDa is shown. The boxes and whiskers are the same as in Fig. 1. Mann-Whitney U test. * p ≤0.050, ** p ≤0.010 and *** p ≤0.001.

Article Snippet: Before that, the membranes were blocked for 1 h at RT with OdysseyR Blocking buffer (LI-COR 927–40000, Lincoln, NE, USA) and then incubated overnight at + 4 ◦C with primary antibodies against synaptic markers SYN-1 (ANR-014, Alomone labs, Jerusalem, Israel, 1:1000 dilution) and SYP (ANR-013, Alomone labs, Jerusalem, Israel, 1:1000), glial marker GFAP (ab7260, Abcam, Cambridge, UK, 1:5000 dilution) and microglial marker IBA1 (PA5-27436, ThermoFisher Scientific, Rockford, IL, USA, 1:700 dilution).

Techniques: Western Blot, Expressing, Membrane, Staining, MANN-WHITNEY

Journal: World Journal of Gastrointestinal Oncology

Article Title: Pancreatic neuroendocrine microtumors in the elderly: A retrospective study using cadaveric pancreatic tissue

doi: 10.4251/wjgo.v17.i12.113198

Figure Lengend Snippet: Histopathological changes of pancreatic neuroendocrine microtumors in Case 4. A: Hematoxylin and eosin staining of pancreatic neuroendocrine microtumors (PNEMTs) of Case 4; B: Immunostaining of PNEMTs in A with synaptophysin (Syn) antibody; C: Immunofluorescence staining of PNEMTs with glucagon (GLU) antibody; D: GLU (green) with insulin (red) double immunofluorescence staining. White asterisk in A: The tumors exhibited an organoid-like architecture, with tumor cells arranged in a trabecular pattern; black asterisk in A: The surrounding pancreatic parenchyma appeared largely normal. Black arrows in A: Indicate a slight membrane structure between the PNEMTs and the pancreatic tissue. Black arrowheads in A: Acinar ductal metaplasia; White arrows in C: GLU-positive cells in the islets of Langerhans; Black and white arrows in D: Insulin-positive cells in PNEMTs and islets of Langerhans, respectively. GLU: Glucagon; Syn: Synaptophysin; Ins: Insulin.

Article Snippet: The primary antibodies used were polyclonal guinea pig anti-human insulin antibody (ready-to-use, IR00261-2J; Dako, Tokyo, Japan), rabbit anti-GLU antibody (1:100, ab92517; Abcam, Tokyo, Japan), mouse anti-human chromogranin A antibody (1:1000, AMAb90525; Atlas Antibodies AB, Stockholm, Sweden), rabbit anti-human synaptophysin (Syn) antibody (1:100, HPA002858; Atlas Antibodies AB, Stockholm, Sweden), and rabbit anti-Ki-67 antibody (1:100, ab16667; Abcam, Tokyo, Japan) diluted in PBS supplemented with 3% bovine serum albumin.

Techniques: Staining, Immunostaining, Immunofluorescence, Double Immunofluorescence Staining, Membrane